Biological Evidence of Improved Wound Healing Using Autologous Micrografts in a Diabetic Animal Model

Background: Tissue healing consists of four main phases: coagulation, inflammation, proliferation, and remodeling. In diabetic patients, this process is stagnant in the inflammatory stage, leading to chronic wounds. The aim study evaluate an animal model biological evidence related use Rigenera® technology (Turin Italy), innovative mechanical procedure isolate autologous micrografts (AMG). Methods: Fifty male Wistar rats were divided into groups: control (C), treated with (CM), (DB), (DBM). experimental setup involved: quantification total collagen elastic fibers; histopathological analysis; immunohistochemical analysis for type I (COL1), III (COL3), vascular endothelial growth factor (VEGF-A), interleukin 4 (IL4) 10 (IL10); evaluation oxidative stress; measurement gluthatione (GSH); and, finally, enzyme-linked immunosorbent assay (ELISA) on tumor necrosis factor-α (TNF-α). Results: AMG induces a faster process: VEGF-A, IL4, IL10, GSH increased, while TNF-α stress decreased. Conclusions: Animals showed more favorable results compared those that did not receive treatment, demonstrating positive participation treatment difficult-to-heal